Fans of Blade Runner have already caught a glimpse of world with super-powered humans secretly living among us, capable of physical feats far beyond your everyday person. But now, with the the CRISPR/CAS9 Gene editing system, are we looking at a future with real replicants? Check out this video to get an inside look at how CRISPR works, and the sorts of wild medical advances are just around the horizon.
CHECK OUT ACS CENTRAL SCIENCE!
Producer: Kirk Zamieroski
Writers: Megan Cartwright & Kirk Zamieroski
Chase Biesel, Ph.D
Martin Jinek, Ph.D.
Roberto Daglio – Mr. Fantastic
Sam Leopard – Back for More
Video interviews with Jennifer Doudna:
Notes from CRISPR talk by Françoise Baylis at AAAS: https://www.evernote.com/l/AJH9UmuXh1VJBp40fv36HDbXD_NnEeifEEU.
Addgene. CRISPR/Cas9 Guide. Web: https://www.addgene.org/CRISPR/guide/.
Addison et al. 2015. Gene Editing and Germ-line Intervention: The Need for Novel Responses to Novel Technologies. Molecular Therapy. Web: http://www.nature.com/mt/journal/v23/n11/full/mt2015185a.html.
Barrangou et al. 2007. CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes. Science. Web: http://science.sciencemag.org/content/315/5819/1709.long.
BBC. 1 February 2016. Scientists get ‘gene editing’ go-ahead. Web: http://www.bbc.com/news/health-35459054.
Chen et al. 2015. Functional disruption of the dystrophin gene in rhesus monkey using CRISPR/Cas9. Human Molecular Genetics. Web: http://hmg.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=25859012.
Deltcheva et al. 2011. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature. Web: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070239/.
Guo et al. 2015. Targeted genome editing in primate embryos. Cell Research. Web: http://www.nature.com/cr/journal/v25/n7/full/cr201564a.html.
Jansen et al. 2002. Identification of genes that are associated with DNA repeats in prokaryotes. Molecular Microbiology. Web: http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2002.02839.x/abstract.
Jinek et al. 2012. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. Web: http://science.sciencemag.org/content/337/6096/816.long.
Komor et al. 2016. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. Web: http://www.nature.com/nature/journal/v533/n7603/full/nature17946.html.
Liang et al. 2015. CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein & Cell. Web: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417674/.
MIT Technology Review. 11 February 2014. Genome Surgery. Web: https://www.technologyreview.com/s/524451/genome-surgery/.
MIT Technology Review. 5 March 2015. Engineering the Perfect Baby. Web: https://www.technologyreview.com/s/535661/engineering-the-perfect-baby/.
Nature. 1 April 2015. Mini enzyme moves gene editing closer to the clinic. Web: http://www.nature.com/news/mini-enzyme-moves-gene-editing-closer-to-the-clinic-1.17234.
Nature. 29 September 2015. Gene-edited ‘micropigs’ to be sold as pets at Chinese institute. Web: http://www.nature.com/news/gene-edited-micropigs-to-be-sold-as-pets-at-chinese-institute-1.18448.
Nature. CRISPR, the disruptor. 3 June 2015. Web: http://www.nature.com/news/crispr-the-disruptor-1.17673.
Science. 17 December 2015. And Science’s 2015 Breakthrough of the Year is… Web: http://www.sciencemag.org/news/2015/12/and-science-s-breakthrough-year.
The Verge. 20 April 2016. Breakthrough method means CRISPR just got a lot more relevant to human health. Web: http://www.theverge.com/2016/4/20/11450262/crispr-base-editing-single-nucleotides-dna-gene-liu-harvard.
Melissa L. Hefferin, Alan E. Tomkinson, Mechanism of DNA double-strand break repair by non-homologous end joining, DNA Repair, Volume 4, Issue 6, 8 June 2005, Pages 639-648, ISSN 1568-7864, http://dx.doi.org/10.1016/j.dnarep.2004.12.005.